Proteome analysis of rat hepatic stellate cells

Transplantation of green fluorescent hepatic stellate cells into rat livers.

THE CURRENT METHOD to treat end-stage hepatic disease is whole-liver transplantation. However, this method is limited mainly by a shortage of organ donors. Therefore, developing effective therapies for hepatic cell transplantation represents a major aim of medical research to relieve the suffering of many patients. Most experimental studies on liver cell transplantation are carried out with adu...

Inhibitory Effect of Tanshinone IIA on Rat Hepatic Stellate Cells

BACKGROUND Anti-inflammation via inhibition of NF-κB pathways in hepatic stellate cells (HSCs) is one therapeutic approach to hepatic fibrosis. Tanshinone IIA (C19H18O3, Tan IIA) is a lipophilic diterpene isolated from Salvia miltiorrhiza Bunge, with reported anti-inflammatory activity. We tested whether Tan IIA could inhibit HSC activation. MATERIALS AND METHODS The cell line of rat hepatic ...

Histone h3 modifications in rat hepatic stellate cells by ethanol.

AIMS Hepatic stellate cells (HSCs) play critical roles in the development of hepatic fibrosis caused by various agents including alcohol. Ethanol causes post-translational modification in histone. The goal of this study is to investigate whether ethanol affected acetylation and methylation of histone H3 in rat HSCs. METHODS We isolated and separated HSCs using collagenase perfusion of liver f...

Hepatic Stellate Cells Preferentially Induce

Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells

To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescenc...